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Test selection
Introduction
GMO detection categories
Levels of quantification
Multiple tests on same sample
Types of tests available
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Introduction
Test selection involves many considerations, including your sample type, the likely sources of GMO, turn-around time needed, buyer requirements and government regulations. Please contact the Export Service Center for assistance in making the most effective and economical decision on which test will meet your specific needs.

GMO detection categories
  1. Cross-species GMO detection determines GMO content from one or more sources of GMO. It is useful for determining GMO content in multiple ingredient products but will not identify the genetically modified species or variety.
  2. Single-species GMO detection provides total GMO content of a single species and is most suitable for products such as grains, flours, soy isolates, etc. where there is a single source of GMO.
  3. Variety-specific GMO detection identifies individual GMO varieties such as StarLink corn.
 
Please call the Export Service Center for more specific information on testing for GM varieties

Levels of quantification
  1. Qualitative analysis reports the presence or absence of GMO material, but will not quantify how much GMO is present.
  2. Single-threshold analysis reports GMO content above or below a single threshold, for example: "greater than 1%" or "less than 1%". The following threshold levels are available: 0.1%, 0.5%, 1%, 2%, 3% or 5% GMO.
  3. Semi-quantitative analysis (multiple threshold) reports GMO content above, below or between two or more thresholds, for example: "greater than 1%", "less than 1% and greater than 0.1%", or "negative at 0.1%". Standard multiple thresholds include 0.1% and 1% GMO, 1% and 5% GMO. Grains may be tested at the thresholds 0.01% and 0.1% GMO.
  4. Real-time quantitative analysis reports precise quantitative results of GMO content. For example, "3% GMO." Real-time quantification is currently only available for corn and soy based products.
 

Multiple tests on same sample
If a sample requires more than one test, the additional testing fees may be reduced if no further DNA extraction is required. When ordering multiple tests, either as an upgrade or concurrently, please call for pricing information.

Types of tests available
1. PCR cross-species GMO detection
These tests simultaneously detect approved GMOs from a variety of species, by detecting genetically modified DNA sequences present in approved GMOs. The predominant genetically modified DNA sequences are 35S Promoter and NOS Terminator. The 35S/NOS/Plus test is able to find all approved GMOs by the detection of two specifically modified DNA sequences present in GMOs not covered by 35S or NOS.
 
One primer set
Primer GMO detection items Levels of quantification
 355 Detects all varieties of GM soy, papaya, rice, sugar beet and zucchini/courgette.
Detects some varieties of GM corn/maize, canola/rapeseed, cotton and potato.
Qualitative
Single threshold
Real-time quantitative
Qualitative and real-time combination (if qualitative is positive and quantification is wanted.)
 
Two primer sets
Primer GMO detection items Levels of quantification
35S/NOS
Detects all varieties of GM corn/maize, soy, papaya, potato, rice, sugar beet, tomato and zucchini/courgette.
Detects some varieties of GM canola/rapeseed and cotton.
Qualitative
Single threshold
35S/GA21
Detects all varieties of GM corn/maize, soy, papaya, potato, rice, sugar beet, tomato and zucchini/courgette.
Detects some varieties of GM canola/rapeseed and cotton.
Qualitative
Single threshold
Real-time quantitative
Qualitative and real-time Combination (if qualitative is positive and quantification is wanted.)
 
Four primer sets
Primer GMO detection items Levels of quantification
35S/NOS Plus two additional GM DNA sequences Detects all varieties of GM corn/maize, soy, canola/rapeseed, cotton, papaya, potato, rice, sugar beet, tomato and zucchini/courgette. Qualitative Single threshold
 

2. PCR single-species GMO detection
PCR tests detect all GM events of a single species (i.e., all corn/maize events, all soy events, etc.). The resulting report is based on the total GM content of the species. A positive test result in a single-species test would not identify which varieties, e.g. types of GM corn, were the source of the GM content. (Note: PCR tests can be expanded to provide additional information, such as the presence of specific varieties. Please call for more information).
 
Types of tests available for single-species GMO detection:
  • Real-time quantitative (currently corn and soy only)
  • Triple-check semi-quantitative
  • Single-threshold
  • Qualitative for GM papaya and zucchini/courgette
  • Qualitative for GM potato
 
GM species available for testing:
  • Canola/rapeseed
  • Corn/maize
  • Cotton
  • Papaya
  • Potato
  • Rice
  • Soy
  • Sugar beet
  • Tomato
  • Zucchini/courgette
 

3. Varietal ID PCR variety-specific GMO detection
Main points:
  1. GMO varieties may be approved in one market, but not in another
  2. Varietal ID detects specific varieties of GMO crops
  3. Varietal ID can be used to detect all commercially available corn varieties
  4. Varietal ID can reduce risk and reduce testing costs significantly
 
Varietal ID is a form of GMO testing used to detect and quantify specific varieties of GMO.
The varietal ID tests are precise, definitive and cost-effective and are designed to specifically detect and quantify GMO varieties that are not approved in a particular country or region.
 
There are two fundamental types of varietal ID for corn.
  • Worldwide varietal ID: The worldwide test can detect all the GMO varieties of corn that have been approved by governments in North and South America. At this time, this list covers virtually all GMO corn varieties approved to date around the world. However, many of these varieties have not been approved for human consumption in other regions of the world. This test is particularly useful when a seller is not sure which country will be the final destination for the product, and desires access to the widest possible market. This test can also be used to rule out particular markets (i.e., if the product is found to contain a variety unapproved in one particular nation or region then it can be sent elsewhere).
  • Region-specific varietal ID: The region-specific test can detect those GM varieties that are not approved in one specific nation or region. Using this more economical test, buyers and sellers can determine if a product is suitable for a particular target market.
 
Both worldwide varietal ID and region-specific varietal ID are available in two configurations.
  • All varieties: This configuration identifies all GM corn varieties that have been approved for production in some nations, irrespective of whether they have been approved elsewhere. This includes both varieties that have been commercialized widely as well as those that have been approved but have not actually been widely commercialized. This test can be done either on a region-specific or a worldwide basis.
  • Commercialized varieties only: When you choose this option, both the worldwide and the region-specific tests limit their detection to only those GM varieties that have been widely grown commercially. Although not as comprehensive as the all varieties option, this option offers a more economical test for the varieties most likely to be present.
 
GMO screening saves you money.
 
To save you money, varietal ID is carried out in a two-phase process. First, we determine if there are any GMOs in your sample. Second, if GMOs are detected in the sample, we continue testing to identify specifically which GMOs are present. When the sample does contain GMOs, the test you ordered will be run to completion. When no GMOs are detected, the second phase is cancelled and you will be billed only for the GMO detection test
 

Return to GMO home page
 

 
Page updated: May 23, 2007

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